GJB2 Knockout cell line (HaCat)

GJB2 Knockout cell line (HaCat) Order

Catalog#

YKO-H1267

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

GJB2 Knockout cell line (HaCaT)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：3~1：5

Species

Human

Gene Name

GJB2

Gene ID

2706

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript GJB2-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HaCaT			STR Info (Cell bank) Cell Line:HACAT
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	10	12		10	12
D7S820	9	11		9	11
D16S539	9	12		9	12
VWA	16	17		16	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	9	11		9	11
D12S391	18	23
FGA	24	24
D2S1338	17	25
D21S11	30.2	30.2
D18S51	12	12
D8S1179	14	14
D3S1358	16	16
D6S1043	12	19
PENTAE	7	12
D19S433	13	14
PENTAD	13	13
D1S1656	11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GJB2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HaCaT cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GJB2 gene knockout GJB2 Knockout cell line (HaCaT) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

GJB2 Knockout cell line (HaCat)

Catalog#

YKO-H1267

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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